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Iwanaka Nobumasa

    The Department of Health and Nutrition　Associate Professor
Last Updated :2023/12/21

Researcher Information

URL

Research funding number

  • 80584002

ORCID ID

Researcher ID

  • AAM-9151-2021

J-Global ID

Research Interests

  • zebrafish   天然有機化合物   機能性食品   脳   骨格筋   グロビンタンパク質   ミトコンドリア   エネルギー代謝   肥満   生活習慣病   シグナル伝達   筋収縮   心拍変動   運動生化学   運動生理学   健康科学   

Research Areas

  • Life sciences / Molecular biology
  • Life sciences / Physiology
  • Life sciences / Sports science
  • Life sciences / Nutrition and health science

Academic & Professional Experience

  • 2022/04 - Today  Kyoto Koka Women's UniversityDepartment of Health and Nutrition / Faculty of Health ScienceAssociate professor
  • 2016/04 - Today  Kyoto University国際高等教育院非常勤講師
  • 2017/04 - 2022/03  Kyoto Koka Women's UniversityThe Faculty of Health Science講師
  • 2016/04 - 2017/03  Kyoto Koka Women's UniversityThe Faculty of Health Science非常勤講師
  • 2014/04 - 2017/03  Kyoto University of EducationFaculty of Education非常勤講師
  • 2015/04 - 2016/03  Ritsumeikan UniversityCollege of Sport and Health Science非常勤講師
  • 2015/04 - 2016/03  Ritsumeikan UniversityCollege of Sport and Health Science客員協力研究員
  • 2014/04 - 2015/03  Ritsumeikan UniversityCollege of Science and Engineering産学連携研究員
  • 2013/04 - 2014/03  Ritsumeikan UniversityCollege of Economics非常勤講師
  • 2012/04 - 2014/03  Ritsumeikan UniversityRitsumeikan-Global Innovation Research Organization専門研究員
  • 2011/04 - 2012/03  富山県立総合衛生学院看護学科非常勤講師
  • 2010/04 - 2012/03  University of Toyama非常勤講師
  • 2010/04 - 2012/03  Kanazawa University人間科学系博士研究員
  • 2009/10 - 2010/03  Kyoto UniversityGraduate School of Human and Environmental Studies非常勤研究員
  • 2009/04 - 2009/09  Kyoto UniversityGraduate School of Human and Environmental Studies技術補佐員
  • 2007/04 - 2009/03  Kyoto UniversityGraduate School of Human and Environmental Studies研修員

Education

  • 2004/04 - 2007/03  Kyoto University  Graduate School of Human and Environmental Studies  Department of Human Coexistence
  • 2002/04 - 2004/03  Kyoto University  Graduate School of Human and Environmental Studies  Department of Human Coexistence
  • 1998/04 - 2002/03  Kyoto Institute of Technology  繊維学部  高分子学科
  • 1990/04 - 1993/03  愛光高等学校

Association Memberships

  • 日本禁煙学会   日本分子生物学会   THE JAPANESE SOCIETY OF NUTRITION AND DIETETICS   

Published Papers

Books etc

  • アンチ・エイジングシリーズ4 進化する運動科学の研究最前線
    岩中 伸壮 (Contributor第1編 運動の基本 第3章 生理機能の分子生物学 第2節 持久性運動能力向上とその分子基盤)株式会社エヌ・ティー・エス 2014/12

MISC

Research Grants & Projects

  • 健康寿命延伸を目指したグロビン蛋白質生合成シグナルの解明
    日本学術振興会：科学研究費助成事業 基盤研究(C)
    Date (from‐to) : 2020/04 -2023/03 
    Author : 岩中 伸壮
  • Japan Society for the Promotion of Science：Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Exploratory Research
    Date (from‐to) : 2015/04 -2018/03 
    Author : Masuda Kazumi; TAKAKURA HISASHI; HASHIMOTO TAKESHI; IWANAKA NOBUMASA
     
    Mitochondrial biogenesis in response to exercise is reliant on adaptive changes through potentially key signal for activating mitochondrial biogenesis (eg. activation of SIRT1, AMPK and PGC-1a). Polyphenol curcumin is a natural antioxidant exhibiting a variety of pharmacological activities and therapeutic properties. However, it remains unknown the effect of curcumin and underlying mechanism on skeletal muscle for regulation of mitochondrial biogenesis. The purpose of the present study was to examine the effects of endurance training (eTR) and curcumin treatment on mitochondrial biogenesis and to identify some of key cascade along the cell signaling. The experimental results suggested that that curcumin treatment with eTR regulated mitochondria biogenesis in skeletal muscle through SIRT1 and AMPK -mediated signaling pathway.
  • Japan Society for the Promotion of Science：Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)
    Date (from‐to) : 2014/04 -2017/03 
    Author : Masuda Kazumi; TAKAKURA HISASHI; IWANAKA NOBUMASA; HASHIMOTO TAKESHI; HAYANO TOSHIYA; KATO YUKIO; HANAI YOSHITERU
     
    The present study focused on physiological interaction of myoglobin (Mb) with mitochondria in myocyte. In order to identify the hypothesis, we established Mb overexpressing cultured cell model. The Mb-overexpressing myocytes consumed oxygen faster and showed higher enzymatic activity especially in activity of complex-IV. The Mb-siRNAs transfection canceled these positive effects observed in Mb-overexpressed myocytes. We further tested about Mb localization by mitochondrial fractionation assay involved serial centrifuges. The Mb was, then, observed in the mitochondrial intermembrane space. These evidences suggest that Mb would be one of the factors underlying the mechanisms to regulate muscle mitochondrial respiration through an interaction with mitochondrial complex.
  • ミオグロビン発現制御機構および生理機能の解明と有酸素性運動能力向上への応用
    日本学術振興会：科学研究費助成事業 若手研究(B)
    Date (from‐to) : 2014/04 -2016/03 
    Author : 岩中 伸壮
     
    平成27年度は、細胞・動物実験によりミオグロビン発現に関わるシグナル経路の検討を実施した。骨格筋細胞株に対し、細胞内cAMP濃度の増加を介しPKAを活性化させるメチルキサンチン類 (カフェイン・テオフィリン)を添加した結果、ミオグロビンの遺伝子・タンパク質発現が有意に増加した。また、ミトコンドリア生合成ならびに脂質代謝に関連する遺伝子発現に関しても、同様に有意な増加が観察された。PKAの阻害剤であるKT5720は、メチルキサンチン類によるミオグロビンの遺伝子発現の増加を抑制した。 細胞実験において、ミオグロビン生合成を制御することが示唆されたcAMP/PKA経路が、生体においてもミオグロビン発現に関与しているかを検討するため、C57BL/6マウスに対するテオフィリンの慢性投与実験を行った。テオフィリンの慢性投与により、体重ならびに脂肪組織重量の有意な減少が見られたが、腓腹筋・足底筋・ヒラメ筋におけるミオグロビンの発現量に関しては有意な増加が見られなかった。 以上から、細胞実験により、cAMP/PKA経路がミオグロビン生合成を制御する新規経路であることが示唆されるが、生体実験において同様の経路が作用し得るかに関しては今後の検討課題である。
  • Japan Society for the Promotion of Science：Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Exploratory Research
    Date (from‐to) : 2013/04 -2015/03 
    Author : KONISHI Satoshi; HASHIMOTO Takeshi; IWANAKA Nobumasa
     
    This study aimed to construct a system for programmable culture, measurement, and control of fat cell on a MEMS chip. We focused on development of the MEMS chip for fat cell culture as well as systemization. A MEMS chip was integrated with supply function of culture medium and chemical solutions. Three layers of polymers were bonded to form wells and channels in a chip. Several culture wells were prepared on a chip of 40mm square. A constructed system was equipped with a controller of supply of culture medium and chemical solutions. It has also become possible to capture microscopic images of cultured fat cell. Measurement results were feedback to control conditions of cultured fat cell through a computer. Real fat cell was successfully cultured and controlled by our system. As a result, developed efficient system for controllable cell culture will contribute to further investigation of fat cell.
  • Japan Society for the Promotion of Science：Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Exploratory Research
    Date (from‐to) : 2013/04 -2015/03 
    Author : MASUDA Kazumi; TAKAKURA Hisahi; IWANAKA Nobumasa; HASHIMOTO Takeshi; HAYANO Toshiya; HANAI Yoshiteru; KATO Yukio; SUGIURA Tomoko
     
    Cellular respiration depends upon a coordinated response of the cardiovasculature and metabolism. Myoglobin (Mb), expressing specifically in myocytes, has been found in mitochondria. This unique finding has led us to the hypothesis that mitochondrial Mb may have a direct role for the regulation of mitochondrial respiration. In order to test this idea, we determined protein-protein interaction between Mb and mitochondrial proteins in genetically modified C2C12 cell by proteomic analysis, IP and WB. It was confirmed that Flag-tagged Mb localized in mitochondria in the C2C12 cells that express Flag-Mb stably. Proteomic analysis identified several mitochondrial proteins, which interacted with Mb. These proteins detected in IP samples of Mb-Flag, occupied 24% of entire proteins. IP and WB detected both outer and inner membrane proteins (VDAC and COX-IV) from the mitochondrial fraction. These results suggested the potential interaction of mitochondrial proteins with Mb in skeletal myocytes.
  • Japan Society for the Promotion of Science：Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B)
    Date (from‐to) : 2011/04 -2015/03 
    Author : IWANAKA Nobumasa; MASUDA Kazumi; HASHIMOTO Takeshi
     
    Oxygen is essential for human activities. It is needed to generate ATP, which is an energy source consumed by mitochondria inside cells. Myoglobin is a type of protein which assists oxygen transport inside muscle cells, from outside to mitochondria, and stores a large amount of oxygen. The objective of this study was to reveal the regulatory mechanism for myoglobin expression and its physiological significance. According to the results of detailed analyses, it was found that elevation of cyclic AMP concentration inside the skeletal muscle cells was crucial to increase myoglobin expression.
  • Japan Society for the Promotion of Science：Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)
    Date (from‐to) : 2011/04 -2014/03 
    Author : MASUDA Kazumi; TAKAKURA Hisashi; IWANAKA Nobumasa; HASHIMOTO Takeshi; HAYANO Toshiya; HANAI Yoshiteru; KATO Yukio
     
    Cellular respiration is influenced by a wide range of mechanisms including the response of the cardiovascular and metabolic systems to meet the changing energy demands in muscle. The present focused on myoglobin (Mb), an important cellular O2 binding protein that is expressed in skeletal and cardiac muscle cells, and set a hypothesis that Mb will have a direct interaction with the mitochondrial respiration. The present study using rodent hindlimb muscles and cultured myoblast (L6), obtained evidences that Mb directly co-localized on mitochondria within a myocytes. Unexpectedly, Mb presences in mitochondria. The mitochondrial Mb can interact with complex IV to facilitated O2 consumption in mitochondria. Further investigation must be necessary to clarify the target proteins of Mb within mitochondria to obtain the new physiological functions of the mitochondrial Mb in myocytes.
  • 骨格筋細胞培養ディッシュの研究開発
    国立研究開発法人 科学技術振興機構（JST）：研究成果最適展開支援プログラム(A-STEP) 探索タイプ
    Date (from‐to) : 2013/08 -2014/03 
    Author : 岩中 伸壮
  • Japan Society for the Promotion of Science：Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Exploratory Research
    Date (from‐to) : 2011 -2012 
    Author : MASUDA Kazumi; IWANAKA Nobumasa; KATO Yukio; SUGIURA Tomoko; HASHIMOTO Takeshi; HANAI Yoshiteru
     
    In skeletal muscle, carnitine is essential for the translocation of long-chain fatty-acids into the mitochondrial matrix for subsequent β-oxidation Based on the concept that increased carnitine availability is beneficial to skeletal muscle metabolic process, a large amount of research was directed towards investigating the effects of carnitine supplementation on exercise performance. However, it has been debated about contribution of carnitine for energy metabolism in skeletal muscle and whether carnitine supplementation can improve physical performance in healthy subjects. In order to resolve the key issues, the present study focused on the carnitine transport mechanism across the skeletal muscle plasma membrane. Due to lack of endogenous synthesis of carnitine in myocytes, skeletal muscles need to import this molecule from blood, suggesting that muscle carnitine uptake is most likely the limiting factor to muscle carnitine availability. The specific carnitine transporter, OCTN2, is expressed in skeletal muscles and is assumed to transport carnitine into myocytes. The present study obtained several scientific evidences that carnitine uptake capacity via the OCTN2 is up-regulated during muscle contraction through the mechanisms of OCTN2 translocation from cytosol toward plasma membrane.
  • ミトコンドリアの呼吸活性に対する酸素輸送担体の発現量の意義
    日本学術振興会：科学研究費助成事業 研究活動スタート支援
    Date (from‐to) : 2010 -2011 
    Author : 岩中 伸壮
     
    本研究は、酸素輸送担体:ミオグロビン(Mb)を過剰発現あるいは発現抑制する骨格筋培養細胞系を確立するとともに、骨格筋細胞内のMb発現量を調節した時のMbとミトコンドリアの相互作用、親和性、ならびに筋細胞の呼吸活性について検証することを目的とした。 本年度は、1)骨格筋芽細胞の選択と筋管への分化条件の検討、2)Mbを過剰発現させるための発現ベクターの作製に取り組んだ。その結果、次のような成果を得た。1)骨格筋培養細胞としてラット由来骨格筋芽細胞(L6)を用いたが、推奨培養条件では筋管形成が十分ではないため、インスリン刺激による分化誘導を試みた。細胞培養液へのインスリン添加を行ったところ、分化が促進され、筋管細胞が増加したL6細胞を得た。また、分化による発現タンパク質の経時変化を観察したところ、インスリン刺激によりミトコンドリアマーカータンパク質の増加が確認された。2)Mbを過剰発現させるため、哺乳類細胞で高レベルの発現を行うCMVプロモーター搭載のpcDNA3ベクターを用い、Mb cDNAをTAクローニングにより組み込んだ。さらに、過剰発現させた際にタンパク質相互作用、親和性を確認するため、エピトープタグであるc-mycを付加し、myc/Mb/pcDNA3発現ベクターを得た。c-mycは免疫反応性の高いエピトープタグであり、大きさが比較的小さいため、Mbのような低分子タンパク質の機能を阻害しにくいと考えられる。 来年度は,作製した発現ベクターを用いて遺伝子導入を行い、Mbを安定して過剰発現するL6骨格筋芽細胞株を作製し、Mbとミトコンドリアとの関連性や呼吸活性の変化の解明に取り掛かる予定である。
  • Japan Society for the Promotion of Science：Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (A)
    Date (from‐to) : 2008 -2010 
    Author : MASUDA Kazumi; HASHIMOTO Takeshi; HANAI Yoshiteru; KANO Yutaka; IWANAKA Nobumasa; KATO Yukio; SUGIURA Tomoko
     
    Cellular respiration is influenced by a wide range of mechanisms including the response of the cardiovascular and metabolic systems to meet the changing energy demands in muscle. The present study focused on myoglobin (Mb) expressed in the skeletal muscle and myocardial cells and hypothesized that Mb would closely associate with mitochondrial respiration during muscle contraction. The experimental evidences using in vivo muscle perfusion technique, suggested that Mb released its binding oxygen to mitochondria at onset of contraction and the response of Mb oxygen releasing was closely related with mitochondrial respiration. Furthermore, the immunohistochemical and immunoprecipitate analysis suggested that a part of Mb directly co-localized on mitochondria within a myocyte.

Academic Contribution

  • 高齢者体力計測事業
    Date (from-to) :2009/04-2010/03
    Role: Planning etc
    Type: Academic research
    京都市社会福祉協議会・老人福祉センター
  • 生活習慣病改善のための運動療法教室
    Date (from-to) :2005/04-2010/03
    Role: Planning etc
    Type: Academic research
    京都大学医学部附属病院内分泌代謝内科
  • 介護予防推進モデル事業（健康増進・運動教室）
    Date (from-to) :2007/04-2009/03
    Role: Planning etc
    Type: Academic research
    京都市社会福祉協議会・老人福祉センター
  • 高齢者介護予防・健康づくり講座
    Date (from-to) :2006/04-2007/03
    Role: Planning etc
    Type: Academic research
    京都市上京老人福祉センター
  • 女性肥満改善教室（きらり☆☆ビューティーアップ教室）
    Date (from-to) :2006/04-2007/03
    Role: Planning etc
    Type: Academic research
    兵庫県丹波市
  • メタボリック・シンドローム改善教室（女性「ビューティアップ教室」、男性「ちょいやせ教室」）
    Date (from-to) :2006/04-2007/03
    Role: Planning etc
    Type: Academic research
    兵庫県篠山市
  • 高齢者向け転倒予防体操プロジェクト
    Date (from-to) :2005/04-2006/03
    Role: Planning etc
    Type: Academic research
    京都市上京老人福祉センター
  • 運動を中心とした健康教室
    Date (from-to) :2005/04-2006/03
    Role: Planning etc
    Type: Academic research
    兵庫県朝来市

Others

  • 2012/04 立命館大学 「立命館イノベーションフェア研究奨励賞(研究機関部門 優秀賞) 」受賞

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